In traditional East Asian medicine, meridians are described as longitudinal pathways connecting acupuncture points through which qi flows. Despite their central role in clinical practice, these pathways are invisible and their biological basis has remained elusive. Our laboratory has previously reported that acupuncture points share similarities with neurogenic spots. The present study investigated the urinary bladder meridian in a rat model of bladder hyperactivity using the neurospot technique. Bladder hyperactivity was induced by continuous intravesical infusion of physiological saline, which reliably evoked rhythmic bladder contractions. When Evans blue dye was intravenously injected, distinct skin regions corresponding to neurogenic spots were revealed and the resulting pattern closely resembled the traditional Foot Taiyang Bladder Meridian. Functional validation was performed by stimulating these Evans blue derived spots with electroacupuncture. Stimulation significantly altered bladder activity, indicating that these sites are functionally relevant to bladder regulation. Moreover, direct stimulation of the spinal nerve 6 (L6) not only suppressed bladder contractions but also induced the appearance of a meridian-like line along the dermatome. This finding supports the hypothesis that the Bladder Meridian corresponds to the somatosensory territory of spinal nerve 6. These results demonstrate that the Foot Taiyang Bladder Meridian may represent a neurophysiological phenomenon associated with specific spinal segmental innervation. This study provides experimental evidence bridging traditional meridian theory with modern neuroanatomy and physiology, and suggests that meridian pathways can be mapped and functionally validated using neurospot-based approaches.